Application

Goat polyclonal Anti-Human IgM (µ-chain specific) antibody may be used to identify and quantitate the level of human IgM in vitro and in human serum or other biological fluids by immunochemical or immunohistological techniques.

Anti-Human IgM (μ-chain specific) antibody is suitable for Ouchterlony double diffusion and quantitative precipitin assay. It was used in B-cell receptor cross-linking studies, AuBioDOT assays, ELISA, protein purification and gel blot analysis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Human serum-derived IgM may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Immunoglobulin M (IgM) antibodies appear early in the course of infections. IgM antibodies are responsible for agglutination of red blood cells in mis-matched blood transfusions. The level of IgM may vary with the status of disease or infection.

IgM antibodies are present as pentamers in the serum and are produced in response to antigens Anti-Human IgM (μ-chain specific) antibody is specific for human IgM with mu chain. The antibody preparation is specific for human IgM when tested against purified human IgA, IgG, IgM, Bence Jones κ and Bence Jones λ myeloma proteins.

Immunogen

Purified human IgM as immunogen

Physical form

Lyophilized from 0.01 M sodium phosphate, 0.015 M sodium chloride, pH 7.2

Reconstitution

Reconstitute with 0.135 M sodium chloride.

Specificity

Goat polyclonal Anti-Human IgM (µ-chain specific) antibody reacts with the m-chain of human IgM. It is specific for human IgM when tested against purified human IgA, IgG, IgM, Bence Jones kappa and Bence Jones lambda myeloma proteins.